Bangladesh
1965

A calf secured for vaccinia virus production. Animals used included calves, sheep, and water buffaloes. They were tranquilized or anesthetized, secured to a special table, shaved and the skin thoroughly cleaned. Skin was scarified in two directions with a sharp-toothed device and vaccinia virus implanted. Animals were penned for 4 days, then humanely killed and the virus-containing pulp collected.